| Abstract Detail
Ethnobotany Idris , Oladayo Amed [1], Wintola, Olubunmi Abosede [2], Afolayan, Anthony Jide [2]. Screening of Rumex crispus extracts potency using Caenorhabditis elegans model: an ultrastructural study of the effect and non-targeted identification of the bioactive compounds. Traditional healers and ethnoveterinary medicines use several medicinal plants such as Rumex crispus L. to treat endoparasite infections. R. crispus has been established by researchers to be effective on a few parasitic worms. In this study, we evaluate the potency of R. crispus extracts on the model organism: Caenorhabditis elegans. The bioactive compounds of the extracts were examined at multiple scales moving from deposited elements, functional groups and compounds identification. Different solvent extracts of the plant were tested against C. elegans for 72 h. The interaction between the external cuticle of C. elegans, the internal organs and the extracts of R. crispus was examined using light microscopy (LM), scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) spectroscopy. Light microscopy and SEM analysis showed damages on the body wall, intense desquamation of the cuticle and shrunk body of the nematode. Fourier-transform infrared spectroscopy (FTIR) spectra reveal various characteristic vibration bands and fingerprint bands at 3400 - 600 cm−1, identifying phenols, organic acids, aromatics, amines among others in the plant. Further compounds identification in R. crispus with Liquid chromatography-mass spectrometry (LC-MS) reveals phytochemicals under the categories; flavonoids, steroidal alkaloid, proanthocyanidin and steroids. In conclusion, this study confirmed R. crispus has anthelmintic potentials, using standardised C. elegans models as a tool and suggest that there could be novel compounds yet to be explored in the studied plant that could be of great benefit to livestock and humans.
1 - North-West University, Zoology , 57 Eleazer Street, Potchefstroom, North-West, 2531, South Africa
2 - Medicinal Plants and Economic Development (MPED) Research Centre, Department of Botany, University of Fort Hare, Alice , 5700, South Africa
Keywords:
Caenorhabditis elegans
gastrointestinal infections
anthelmintic novel drug
bioactive compounds
phytochemical
ethnoveterinary medicines .
Presentation Type: Oral Paper
Session: ETH1, Ethnobotany
Location: Virtual/Virtual
Date: Tuesday, July 28th, 2020
Time: 3:30 PM
Number: ETH1003
Abstract ID:93
Candidate for Awards:None |
