Abstract Detail


Mendez-Reneau, Jonas [1], Sigel, Erin [1].

Comparative Phylogenetic Analysis of Rapid Divergences and Reticulations in the Fern Genus Polypodium s.s. using Target-Capture Methods.

We present a case study using Target-Capture methods for inferring phylogenetic relationships in Polypodium s.s. (Polypodiaceae), a group with several rapidly diverged lineages and a propensity for hybridization. Using data generated by the GoFlag Project and a custom bioinformatic pipeline, we retrieve nearly complete plastid genomes and phased alleles or homeologs for approximately 370 low-copy nuclear markers. We then compare analytical approaches to assess evolutionary hypotheses in Polypodium s.s. using concatenated consensus-sequence supermatrices for nuclear and plastid data, as well as alignments comprising multiple alleles or homeologs per locus per individual. We estimate divergence times among major clades and species, including a preliminary sampling of known allopolyploids. We recover strong support for the monophyly and sister relationships of major lineages within Polypodium s.s. and strong support for previously unresolved species-level relationships. Comparison of phylogenetic approaches reveals higher resolution for phased single phased nuclear marker approaches versus concatenated consensus sequence supermatrix approach, particularly in the case of allopolyploid taxa that are dependent on phasing to identify homeologs.

1 - University of Louisiana, Lafayette, Biology, 410 E Saint Mary blvd, Lafayette, LA, 70503, United States

cryptic species

Presentation Type: Oral Paper
Session: PTER1, Pteridology I
Location: Virtual/Virtual
Date: Monday, July 27th, 2020
Time: 11:00 AM
Number: PTER1005
Abstract ID:128
Candidate for Awards:Edgar T. Wherry award

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