| Abstract Detail
Comparative Genomics/Transcriptomics Landis, Jacob [1], Valderrama, Eugenio [2], Grunder, Nikolaus [3], Skinner, David [4], Sass, Chodon [5], Vargas , Maria Pinila [6], Guan, Clarice [6], Almeida, Ana Maria [7], Specht, Chelsea [8]. The genomic signatures of convergent evolution of pollination syndromes in Costus (Costaceae). The angiosperm genus Costus L., also known as the spiral gingers, is comprised of roughly 80 species with a widespread distribution through tropical and subtropical regions of Africa and the Americas and accounts for much of the species diversity within the family Costaceae Nakai. Throughout the genus there is a diversity of floral forms associated with pollinator attraction and pollination efficiency. Phylogenetic analyses have identified multiple transitions from insect to bird pollination across the genus, associated with strikingly consistent shifts in floral morphology. Using a combination of sequencing approaches, we have generated 80X short-read data from Illumina and around 20x long-read coverage from Oxford Nanopore for 10 species pairs for de novo genome assembly. Even though we have not achieved chromosome level assemblies, we are recovering at least 80% of the embryophyta BUSCO genes, giving confidence in comparative analyses. Downstream comparative genomic analyses allowed for identification of genomic regions associated with shifts in pollinator and repeated transitions potentially involving the same locus (or loci) with differing regulatory constraints. Candidate genes associated with flower color, flower symmetry and organ size have been analyzed for the 20 targeted species as well as an additional 47 species across the genus with known pollinator types for large-scale analyses for signatures of selections in genes associated with the identified transitions in floral morphology.
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1 - Cornell University, Plant Biology and the L.H. Bailey Hortorium, School of Integrative Plant Science, 506 Mann Library Building, Ithaca, NY, 14850, USA 2 - Cornell University, Plant Biology, CALS, 510 Mann Library, Cornell University, Ithaca, NY, 14853, United States 3 - Cal State East Bay 4 - Other, FL 5 - Plant And Microbial Biology, 111 Koshland Hall, Berkeley, CA, 94720, United States 6 - Cornell University, School of Integrative Plant Sciences 7 - California State University East Bay, Department Of Biology, 25800 Carlos Bee Blvd, Hayward, CA, 94542, United States 8 - Cornell University, School of Integrative Plant Science and L.H. Bailey Hortorium, 502 Mann Library, Ithaca, NY, 14850, USA
Keywords: genome assembly insect pollination bird pollination comparative genomics pollination syndromes signatures of selection.
Presentation Type: Oral Paper Session: CG1, Functional & Comparative Genetics/Genomics I Location: Virtual/Virtual Date: Tuesday, July 28th, 2020 Time: 1:00 PM Number: CG1003 Abstract ID:196 Candidate for Awards:Margaret Menzel Award |