Abstract Detail

Angiosperms353: A new essential tool for plant systematics

On the potential of Angiosperms353 for population genomics.

Assessing genetic diversity, admixture, and demographic history in non-model organisms is frequently hindered by the high costs of marker development and sequencing. Recent advancements in universal probe sets, such as Angiosperms353, suggest a potential role for target capture in population genetics. Target capture has some advantages over other molecular techniques commonly used for population genetics in plants. For example, orthology can be more easily determined without a reference genome, missing data is typically minimized, and sequence recovery is increased for the degraded DNA of herbarium specimens. However, the potential for Angiosperms353 to be used within species is unproven, particularly regarding sequence variability within species and appropriate data analysis workflows. Here I discuss pilot projects that explored both of these issues using sequences recovered within species and species complexes. When paired with protocol modifications aimed at substantially reducing per-sample costs, the results indicate a strong potential of Angiosperms353 to be a useful tool at and below the species level.

1 - Texas Tech University, Biological Sciences, 2901 Main Street, Ms3131, Lubbock, TX, 79409, United States

Keywords:
target capture
Angiosperms353
HybSeq
Conservation genomics.

Presentation Type: Symposium Presentation
Session: SY4, Angiosperms353: A new essential tool for plant systematics
Location: Virtual/Virtual
Date: Thursday, July 30th, 2020
Time: 1:30 PM
Number: SY4008
Abstract ID:263
Candidate for Awards:None