Abstract Detail

Phylogenomics

Yardeni, Gil [1], Hess, Jaqueline [2], de La Harpe, Marylaure [3], Barfuss, Michael H. J. [4], Viruel, Juan [5], Paris, Margot [6], Groot Crego, Clara [1], Till, Walter [1], Leroy, Thibault [1], Paun, Ovidiu [7], Lexer, Christian [1].

A target capture approach for exploring the drivers and constraints of adaptive radiation in tillandsioid bromeliads (Tillandsia spp).

The study of adaptive radiation calls for a synthesis between macro-evolutionary and population genetic approaches to uncover the genomic substrate and mechanisms of biological diversification at different time scales. The species-rich and ecologically diverse Bromeliaceae family provides an excellent system for studying the drivers and constraints of adaptive radiation. We focus on Tillandsia, a particularly young subgenus (~6 Mya), yet the largest and most diverse within bromeliads, with over 600 species, extensive geographical ranges and a variety of key adaptations such as epiphytism and divergent photosynthetic and pollination syndromes. We developed a taxon-specific target sequence capture set for bromeliads, specifically created to address hypotheses on neutral processes (tracked via demographic modeling) and the dynamics of adaptive and deleterious variation during a textbook adaptive radiation. The capture set targets over 1,700 coding regions, including single copy and relatively conserved genes, as well as genes putatively involved in several key traits, i.e. genes that may have experienced positive selection and played an important role in the explosive evolutionary radiation. The design allowed us to include both single-copy and multi-copy genes. To assess the utility of our capture set, we compare it to a ‘universal’ Angiosperm probe set (Angiosperm-353), examining the power of both sets in resolving the phylogenomic relationships within recent radiations and for population genetics inferences of admixture and introgression. We show that the taxon-specific set results in high enrichment success across the entire family, extending significantly into flanking regions of exons to provide information from genetically diverse regions. While both bait sets offer abundant data for phylogenomic inference, the taxon-specific set resulted in higher resolution of phylogenomic relationships in recently radiated taxa and provided insights into the genetic processes accompanying adaptive radiation in Tillandsia. We will further discuss the benefits and limitations of the approach.

1 - University of Vienna, Department of Botany and Biodiversity Research, Rennweg 14, Vienna, 1030, Austria
2 - Helmholtz Centre for Environmental Research - UFZ, Department of Soil Ecology, Theodor-Lieser-Str. 4, Halle/Saale, 06120, Germany
3 - University of Zürich, Department of Geography, Winterthurerstrasse 190, Zürich, 8057, Switzerland
4 - University Of Vienna, Botany And Biodiversity Research, Rennweg 14, Vienna, 1030, Austria
5 - Royal Botanic Gardens, Kew, Conservation Science, Kew, Richmond, London, England
6 - University of Fribourg, Department of Biology, Route Albert-Gockel 3, Chemin du Musée 10, Fribourg, 1700, Switzerland
7 - University Of Vienna , Department Of Botany And Biodiversity Research, Rennweg 14, Vienna, A-1030, Austria

Keywords:
Bromeliaceae
Targeted sequencing
Tillandsia
population genomics
phylogenomics
adaptive radiation.

Presentation Type: Oral Paper
Session: PHYL1, Phylogenomics I
Location: Virtual/Virtual
Date: Monday, July 27th, 2020
Time: 1:45 PM
Number: PHYL1006
Abstract ID:277
Candidate for Awards:None