Abstract Detail

Evolutionary Developmental Biology (Evo-Devo)

Dunivant, Taryn [1], Singh, Vibhuti [2], Hileman, Lena [3].

Investigation of the function and expression of CYCLOIDEA genes in Mimulus lewisii.

Angiosperm diversity has been largely attributed to numerous independent transitions between radial and bilateral flower symmetry. Examination of the genetic controls for bilateral flower symmetry in the model system Antirrhinum majus has revealed that the paralogs CYCLOIDEA (CYC) and DICHOTOMA (DICH) have partially redundant functions in establishing dorsal specific flower identity. A similar pattern holds in Mimulus, where a unique duplication event in the CYC gene lineage resulted in the paralogs Mimulus-CYC1 and Mimulus-CYC2. Preliminary evidence in Mimulus guttatus has shown that there is functional redundancy between MgCYC1 and MgCYC2 where dorsal flower identity is only lost in lines co-suppressing both paralogs. This suggests that each gene alone is not responsible for the floral symmetry program, but rather, both genes together strongly influence dorsal flower identity. It should be noted that results from Virus Induced Gene Silencing (VIGS) experiments, like those from M. guttatus, can be difficult to characterize due to varying levels and mosaic patterns of gene silencing. In this study, we aim to determine if the floral symmetry program in Mimulus lewisii, a close relative of M. guttatus, has conserved function in comparison to closely related A. majus. We are leveraging newly developed Agrobacterium-mediated stable transformation in the emerging model M. lewisii, to fully characterize the function and expression of MlCYC1 and MlCYC2 by RNA interference (RNAi). With stable MlCYC1 and MlCYC2 silenced lines, we will test for conservation of dorsal flower patterning function and for genetic redundancy. Preliminary results show similar findings to M. guttatus with support for complete functional redundancy between MlCYC1 and MlCYC2. Generating and characterizing MlCYC1;MlCYC2 double RNAi silencing lines will be key to determining if together MlCYC1 and MlCYC2 function fully to determine dorsal identity during M. lewisii flower development.

1 - University of Kansas, Ecology and Evolutionary Biology, 1200 Sunnyside Ave, Lawrence, KS, 66045, USA
2 - University of Kansas, Ecology and Evolutionary Biology, 1200 Sunnyside Ave, Lawrence, KS, 66045, United States
3 - University Of Kansas, Ecology And Evolutionary Biology, 1200 Sunnyside Avenue, Lawrence, KS, 66045, United States

Mimulus lewisii
flower symmetry
stable transformation.

Presentation Type: Poster
Session: P, Evolutionary Developmental Biology (Evo-Devo) Posters
Location: Virtual/Virtual
Date: Tuesday, July 28th, 2020
Time: 5:00 PM Time and date to be determined
Number: PEV004
Abstract ID:322
Candidate for Awards:None

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