Abstract Detail

Ecophysiology

Davis, Rebekah [1], Davis, Kaleigh [2], Mason, Chase [2].

Capillary Electrophoresis Buffer Optimization for Plant Secondary Metabolite Analysis.

Capillary electrophoresis (CE) is an analytical chemistry approach that allows for the efficient separation of metabolites by size and charge of molecules. The goal of this work was to optimize a buffer system for plant tissue analysis using micellar electrokinetic chromatography (MEKC), and by doing so, to understand the role of buffer components in the performance of this form of capillary electrophoresis. In this experiment we implemented a factorial design to optimize buffer composition for separating plant tissue and secondary metabolites. The results of this experiment will be used to optimize a universal buffer for MEKC analysis that can be used on any variety of plant tissues.  To determine the feasibility of this, a diverse set of plant secondary metabolite chemical standards in solution were tested as well as Helianthus annuus tissue to confirm the separation in a real biological sample. The results of this optimization yield insights into the utility of buffer components like electrolyte, surfactant, pH, and additives for MEKC separation.

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1 - University of Central Florida, Biology, 8215 Sun Spring Circle, Unit 62, Orlando, FL, 32825-4717, United States
2 - University Of Central Florida, Department Of Biology, 4110 Libra Dr, Orlando, FL, 32816, United States

Keywords:
Capillary Electrophoresis
Plant Tissue
Secondary Metabolites
Phytochemical Analysis.

Presentation Type: Poster
Session: P, Ecophysiology Posters
Location: Virtual/Virtual
Date: Tuesday, July 28th, 2020
Time: 5:00 PM Time and date to be determined
Number: PPE002
Abstract ID:332
Candidate for Awards:Physiological Section Physiological Section Li-COR Prize