Abstract Detail

Functional Genetics/Genomics

Mosquera, Veronica [1], Maughan, Peter [2], DeTemple, Joseph [3], Whipple, Clinton [4].

Genetic regulation of anthocyanin biosynthesis in flower color in Gilia.

We investigated the genetic regulation of flower color polymorphism as an interesting test case for Gilia (Polemoniaceae) as a model for evo-devo in wild species. An F2 mapping population of 189 individuals was created from two closely related species of leafy-stemmed gilia:   G. capitata is a widespread species found from Baja California to British Columbia with a tight capitate inflorescence and lilac corolla color, while G. yorkii is endemic to the Monarch Wilderness of the Sierra Nevadas and has a much more open inflorescence and white corolla color. Despite their distinctive morphologies, these species formed a fertile hybrid F1 with no obvious meiosis problems capable of generating segregating F2 progeny. The G. yorkii genome was sequenced with PacBIO, polished with Illumina reads, and assembled into contigs. Hi-C reads were used to scaffold the contigs to the chromosome level. The genome size is 2.75 Gbp with 9 chromosomes. Genotyping by Sequencing (GBS) reads of 189 F2 individuals were aligned to G. yorkii reference genome, and Single Nucleotide Polymorphism (SNP) calling identified a set of 73,482 polymorphic SNP sites. We also phenotyped flower color in the F2, and observed data showed a segregation ratio of 3 : 1 between pigmented vs white flowers, respectively, a Chi-square goodness of fit test (X2) was calculated and value was 1.75, inferior to the critical one (X21df ;α 0.05=3.84), suggesting that pigmented flowers is controlled by a  dominant single locus and white flower color is due to a recessive genotype. In addition, a preliminary marker association indicates tight linkage on chromosome 1, near a candidate gene for anthocyanin biosynthesis.

Related Links:
eFLOWER: A framework for understanding the evolution and diversification of flowers

1 - Brigham Young University, Biology, 4102 Life Science Building (LSB), Provo, UT, 84602, USA
2 - Brigham Young University, Plant and Wildlife Sciences, 4125, Provo, UT, USA, 84602
3 - Brigham Young University, Biology, 4102 LSB, Provo, UT, 84602, USA
4 - Brigham Young University, Biology, 4102 LSB, Provo, UT, USA, 84602

Flower color
Single gene

Presentation Type: Poster
Session: P, Functional Genetics and Genomics Posters
Location: Virtual/Virtual
Date: Wednesday, July 29th, 2020
Time: 5:00 PM Time and date to be determined
Number: PCG001
Abstract ID:351
Candidate for Awards:None

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