Abstract Detail



Mechanisms of rapid adaptation through the expression of “heterogenomicity”

Notaguchi, Michitaka [1].

Study on mechanism of hetero grafting.

Plant grafting has been an important technique in agriculture to propagate clones and to obtain benefits of certain rootstocks. However, plant grafting has a limitation in combinations to graft. In general, it has been thought that grafting can be done successfully between the same species, genus, and family, but not between different families because of graft incompatibility. Recently, we found that a genus, Nicotiana, showed an extreme capability of grafting. Nicotiana can be grafted with a wide range of vascular plants, including herbaceous and woody plants. To investigate the molecular basis of Nicotiana interfamily grafting, we performed time-course transcriptome analysis of grafting region. First, we observed upregulation of the genes related to cambium, provasculature and xylem formation and no upregulation of phloem related genes. These gene expression patterns are coincident with morphological observation at the graft junction where parenchyma tissues were proliferated and xylem connection were established, whereas no phloem connection was observed. Second, by comparing transcriptome of Nicotiana interfamily grafting and other graft combinations, we identified characteristic upregulation of several genes related to cell wall modification which was coincident with partial digestion of cell walls at the graft boundary. We observed plasmodesmata formation at the digested cell wall regions resulting in symplasmic transport of RNAs and proteins. Finally, we tested the necessity of such genes in interfamily grafting using a virus induced gene silencing system. We will present these data and discuss about the characteristic features of grafting.


1 - Nagoya University, Bioscience and Biotechnology Center, B414, Graduate School of Bioagricultural Sciences, Nagoya University,, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan

Keywords:
Grafting
Nicotiana
Interfamily grafting.

Presentation Type: Colloquium Presentations
Number: COL06005
Abstract ID:359
Candidate for Awards:None


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