Abstract Detail

Population Genetics/Genomics

Rosati , Ethan M [1].

Undergraduate Student Research Conducting Barcoding and Comparison of DNA Extractions of ITS Region Between Angiosperm and Gymnosperm Herbarium Specimens.

Several goals in this pursuit are to gather and collect the genetic information and lay the groundwork for future engaged learning opportunities. Compare angiosperm and gymnosperm extraction quality and quantity from exsiccate herbarium specimens. The DNA sequences collected will create a reference collection building a valuable student resource for future phylogenetic work. DNA was collected by way of destructive method of grinding. After material collection DNA extraction kits were used. We amplified the DNA via PCR then observed the quality and quantity of the PCR products though gel electrophoresis and nanodrop. To investigate success in amplification of the target DNA sequence the ITS region was sequenced (Cheng, 2016). Geneious software was used to edit DNA and measure quality to determine whether the two clades produced good DNA yield. Ultimately the goal is to add our sequenced data to the barcoding body of knowledge to help biologists have intellectual control, conserve and protect the biota of Utah and the world.

1 - Utah Valley University, Biology, 800 W University Pkwy, Orem, UT, 84058, United States

Keywords:
DNA comparison.

Presentation Type: Poster
Session: P, Population Genetics/Genomics Posters
Location: Virtual/Virtual
Date: Thursday, July 30th, 2020
Time: 5:00 PM Time and date to be determined
Number: PPG001
Abstract ID:44
Candidate for Awards:None