Abstract Detail

Reproductive Processes

Friedman, Jannice [1], Leydet, Karine [2].

Do plants pick their partners post-pollination: Investigating mate preferences and non-random mating in the monkeyflower Mimulus guttatus.

The potential for post-pollination selection has been demonstrated in some plants, and could include sexual selection and/or mate-dependent preferences. However, because plants are internal fertilizers, studies have been limited in identifying the stages at which particular male gametophytes outperform others, and whether female preferences for particular genotypes influence the outcome of fertilization. Here we use an experimental approach that combines in-vivo pollination with an in-vitro assay to visualize growing pollen tubes as they emerge from the style and interact with ovules. We use a fully factorial design with pollination and mating among eight genotypes from a single annual population of the wildflower Mimulus guttatus (syn. Erythranthe guttata). Using replicated crosses, we decompose post-pollination processes to identify pollen grains on stigmas, pollen tubes emerging from styles, pollen tube growth towards ovules, successful targeting between the ovule and pollen tube, and seed set. We find the length of the style on the pollinated plant is significantly associated with successful targeting (ie. fertilization). We discover patterns of non-random mating and identify particular combinations of genotypes that perform better than others, indicating the potential for non-random fertilization. This approach may provide evidence that styles and ovules can exercise choice in selecting pollen grains for fertilization.

1 - Queen's University, Biology Department, Biosciences Complex , 116 Barrie Street, Kingston, ON, K7L 3N6, Canada
2 - Syracuse University, Biology, 106 College St, Syracuse, NY, 13244, USA

Keywords:
sexual selection
female choice
male competition
pollen tubes
ovule
fertilization.

Presentation Type: Oral Paper
Session: REP3, Reproductive Processes 3
Location: Virtual/Virtual
Date: Thursday, July 30th, 2020
Time: 10:00 AM
Number: REP3001
Abstract ID:492
Candidate for Awards:None