Abstract Detail

Comparative Genomics/Transcriptomics

Qaisar, Uzma [1], Imran, Mahnoor [2], Bano, Shaher [2], But, Maria [2].

Involvement of Gbwri1 in fiber development and fatty acid biosynthesis in Gossypium barbadense.

Cotton is the major natural fiber producing crop and Pakistan holds the fourth position in world cotton producers after China, USA and India. Three cultivated species of cotton are Gossypium hirsutum (Upland cotton), G. barbadense (Pima cotton) and G. arboreum (Desi cotton).  G. barbadense produces elite fiber but the yield of this genotype is lower as compared to G. hirsutum which produces medium quality fibers. Fiber transcriptome of G. barbadense was compared with the transcriptome of G. hirsutum and G. arboreum. Expression pattern of ethylene responsive transcription factor Gbwri1 showed close association with fiber lengths and showed significantly higher expression in G. barbadense as compared to G. hirsutum and G. arboreum during fiber development. GBWRI1 possesses two AP2 DNA biding domains through which it binds with the AW-boxes in the promoters of target genes and regulates their expression. Potential target genes of GBWRI1 were identified in cotton through identification of AW- boxes in the promoter sequences of co-expressed genes. The binding of promoter DNA to the GBWRI1 transcription factor was confirmed through EMSA. The target genes of GBWRI1 encode the enzymes which catalyze the steps of fatty acid biosynthesis. Gbwri1 was isolated and transformed in A. thaliana mutant plants through Agrobacterium mediated transformation. In the transgenic lines of A. thaliana, the ectopic expression of GBWRI1 transcription factor resumed the seed weight and seed area to the wild type and rescued the wrinkled phenotype of mutants by resuming the expression of fatty acid biosynthesis genes bccp2 and kas1. GBWRI1 was able to regulate the conversion of fatty acids to sucrose for normal seedling establishment on sucrose free medium. Our results showed that the expression of GbWRI1 in A. thaliana wri1-3 mutant is able to complement impaired phenotype.

1 - SBS, Quaide Azam Campus, University Of The Punjab, Lahore, PB, 54000, Pakistan
2 - University of the Punjab, SBS, Quaide Azam Campus, University of the Punjab, SBS, Quaide Azam Campus, University of the Punjab, Lahore, PB, 54000, Pakistan

fattyacid biosynthesis
fiber development.

Presentation Type: Oral Paper
Session: CG1, Functional & Comparative Genetics/Genomics I
Location: Virtual/Virtual
Date: Tuesday, July 28th, 2020
Time: 1:15 PM
Number: CG1004
Abstract ID:54
Candidate for Awards:None

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