Abstract Detail

Comparative Genomics/Transcriptomics

Kafrawi, Rizky [1], Livshultz, Tatyana [2], Fishbein, Mark [3], Straub, Shannon [1].

Intracellular Gene Transfer Dynamics in Asclepiadoideae (Apocynaceae).

Intracellular gene transfer (IGT) of plastid DNA into mitochondrial genomes is a well-documented occurrence in the field of plant organellar genomics; however, the reverse phenomenon is much less frequently observed in plastid genomes. In this study, a mitochondrial DNA insertion was identified in the plastome sequences of Rhyssolobium dumosum, Hoya multiflora, and Dischidia albida (Marsdenieae). The mitochondrial insert was located in the rps2-rpoC2 spacer of the large single copy region and ranged between 2,447 and 4,646 bp. It was determined to be homologous to the mitochondrial insertion observed in the plastomes of species of Asclepiadeae and Eustegieae in a previous study, which suggests that the IGT event occurred in the common ancestor of Asclepiadeae, Eustegieae, and Marsdenieae, early in the evolution of the milkweed subfamily Asclepiadoideae. Furthermore, phylogenetic analysis of plastid and mitochondrial rpoC2 paralogs revealed that at least four gene conversion events of the mitochondrial copy have taken place in different lineages of Asclepiadoideae. These results support the notion that the interactions between mitochondrial and plastid genomes are dynamic over evolutionary time.

1 - Hobart and William Smith Colleges, Department of Biology, 300 Pulteney Street, Geneva, NY, 14456, USA
2 - Academy Of Natural Sciences Of Drexel University, Department Of Botany, 1900 Benjamin Franklin Parkway, Philadelphia, PA, 19103, United States
3 - Oklahoma State University, Dept Of Plant Biology, Ecology & Evolution, 301 Physical Science, Stillwater, OK, 74078, United States

Keywords:
intracellular gene transfer
gene conversion
plastome
Mitochondrial genome
Asclepiadoideae
Apocynaceae.

Presentation Type: Poster
Session: P, Comparative Genomics and Transcriptomics Posters
Location: Virtual/Virtual
Date: Monday, July 27th, 2020
Time: 5:00 PM Time and date to be determined
Number: PGT002
Abstract ID:587
Candidate for Awards:None