Abstract Detail

Symbioses: Plant, Animal, and Microbe Interactions

Hernández Álvarez , Gustavo Ramiro [1], Mondragón-Chaparro , Demetria [2], Robles, Celerino [3].

Effect of aqueous extracts of oak bark on germination of Alamania punicea Lex (Orchidaceae).

One of the factors that affect the germination of epiphytic orchid seeds is the composition and concentration of chemical compounds present in the cortex of the phorophytes. In a holm oak of the Tooxi community in Oaxaca (Mexico), we record that A. punicea grows abundantly on Quercus rugosa and Q. martinezii, and sparsely on Q. castanea. To explore if such variation could be explained by the effect of the diffusible chemicals of the bark of these host trees on the germination of A. punicea, we selected six trees per species, from which a sample of 5 cm2 of bark was taken. The barks were dried and ground; 100 g per species of tree was taken to which 200 mL of distilled water was added and put in constant agitation per 24 h. Subsequently, three dilutions of each extract (0.1, 1 and 100 µL/mL) were performed. In test tubes with Knudson C medium, 50 already sterilized A. punicea seeds were sown, at each tube we added 100 µL of extract, ten repetitions were made per dilution per host tree, plus a control. We record seed germination following the stages established by Dressler (1981). The percentage of each germination stage was obtained, which were transformed and compared by two-way ANOVA and the Tukey post hoc test. All treatments passed to E0 in percentages below 9%, with the highest transition for Q. rugosa 1 µL/mL; for E1 transition were greater than 90% in all cases, with no differences among species or dilutions, except for Q. martinezzi at 100 mL/L, which presented the lowest percentage of passing; the opposite for E3 where Q. martinezzi at 100 µL/mL  present the highest transition values, the rest of the treatments do not present statistical differences with transition values lowest than 17%; for E3 only the treatments of Q. rugosa 0.1 µL/mL, and Q. martinezzi 1 and 100 µL/mL present transitions with percentages lowest than 1.7%. Our results show that both the host species and the dilutions of the extracts influence germination of A. punicea, where the extracts of Q. rugosa and Q.  martinezzi, the host with high abundance of individuals of A. puniceae, allow all phases of germination. 

1 - Instituto Politécnico Nacional , Oaxaca, Oaxaca, 71233
2 - Instituto Politécnico Nacional, Laboratorio Epífitas Vasculares
3 - Instituto Politécnico Nacional, Laboratorio de Suelos

Chemical Ecology

Presentation Type: Oral Paper
Session: SYMB1, Symbioses: Plant, Animal, and Microbe Interactions I
Location: Virtual/Virtual
Date: Tuesday, July 28th, 2020
Time: 12:30 PM
Number: SYMB1001
Abstract ID:619
Candidate for Awards:None

Copyright © 2000-2020, Botanical Society of America. All rights reserved