Abstract Detail

Bryology and Lichenology

Dou, Jiancong [1], Zhang, Shijia [1], Shen, Fengjiao [1], Niu, Jingyuan [1], Li, Lin [1], Shi, Shuo [2], Zhao, Jiancheng [1].

The Development and Evaluation of DNA Barcodes in Mosses (Bryophyta): Based on Whole-genome Chloroplast Data.

Mosses (Bryophyta), are significant taxa in higher plants and have broad applications in many fields. However, the individuals of mosses are small and often mixed and clustered with different species. The identification is troublesome, and fewer professional researchers keep on engaging in mosses identification work. DNA barcoding has gradually become an effective method to assist mosses identification. However, most of these DNA barcodes came from the researches of angiosperms, which had problems of weak universality or low resolution. In this study, we tried to screen DNA barcodes from the mosses chloroplast genome level, based on the public databases and the genome data produced in this study. Firstly, 12 candidate DNA barcodes of mosses, acquired from 34 chloroplast genome data, were selected. The universal primers at both ends were designed. Then, the candidate barcodes were verified by two ways verification, primer universality, and DNA barcodes resolution, with selected samples of mosses. To verify the universality of primers, 38 samples, which involved 31 species in 22 families of 14 orders from mosses, were selected for DNA extraction and PCR amplification. The results showed that the amplification success rate of all the twelve pairs primers were over 90%, five pairs of them reached 100%. The samples for resolution verification contained 14 species in eight genera of two families in Bryales. Based on the results of ML, BI, and NJ trees constructed by single gene fragment, it showed that the species resolution of ycf2, ycf1,matK, and clpP fragments were better than other barcodes, and their support rates of species branch were all more than 80%. Both the universality amplification verification and the species resolution support that the new DNA barcodes selected are high-quality barcodes suitable for mosses in this study. Among them, matK is a universal DNA barcode in mosses, but the new primers designed in this study had a higher amplification success rate. Some studies showed that ycf1 and clpP contain more genetic variation sites in the chloroplast genome of angiosperms, and ycf2 has not been reported as a universality DNA barcode and widely used. Further research should be conduct on more taxa mosses samples, and the reference database of the DNA barcodes was needed to be established for the application of these DNA barcodes.

1 - Hebei Normal University, College of Life Sciences, No.20, East Nanerhuan Road, Shijiazhuang, Hebei, 050024, China
2 - Hebei Normal University, College of Life Sciences, No. 20 East Nanerhuan Road, Shijiazhuang, Hebei, 050024, China

Keywords:
mosses
Chloroplast Genome
DNA barcoding
Primer Design.

Presentation Type: Poster
Session: P, Bryology and Lichenology Posters
Location: Virtual/Virtual
Date: Monday, July 27th, 2020
Time: 5:00 PM Time and date to be determined
Number: PBL002
Abstract ID:690
Candidate for Awards:None