| Abstract Detail
Systematics Bhatti, Maryum [1], Lee, Aaron [2], Rahman-Vyas, Hiba [1], Howarth, Dianella [3], Donoghue, Michael [4], Clement, Wendy [2]. Combining benchwork and bioinformatics to reconstruct the evolutionary history of CUP-SHAPED COTYLEDON in honeysuckles and relatives. The honeysuckles (Lonicera, Caprifoliaceae, Dipsacales) exhibit a wide array of fused organs that can involve leaves, bracts, bracteoles, and petals. The natural variation of fusion among species of Lonicera make this a well-suited system to study evolution and development of fusion. Past studies have demonstrated that CUP-SHAPED COTYLEDON (CUC) genes regulate fusion of leaves and floral organs in model plant species. Additionally, NAC transcription factors, that together with CUC comprise NAC subfamily 1a, may also play a role in aspects of organ fusion. The goal of this project was to reconstruct a gene tree for NAC subfamily 1a. Specifically, we isolated CUC and NAC genes from species of Dipsacales for which no genomic data were available. We designed degenerate primers from known CUC sequences that successfully amplified both CUC and NAC. Multiple copies were separated by cloning before sequencing. We then combined these data with sequences recovered from genome skimming and transcriptomes to reconstruct a gene tree of NAC subfamily 1a. We recovered 24 sequences of CUC and NAC that confirmed a duplication of CUC2 and helped to pinpoint its origin within the Dipsacales. Ultimately, combining Sanger sequencing with genomic data was critical to sampling the full phylogenetic diversity of Dipsacales in a first step toward understanding the role of CUC and NAC in regulating fusion in Lonicera.
1 - The College of New Jersey, Biology, 2000 Pennington Road, Ewing, NJ, 08628, United States
2 - The College of New Jersey, Department of Biology, 2000 Pennington Road, Ewing, NJ, 08628, USA
3 - St. John's University, Department Of Biological Sciences, St. Albert Hall Rm 257, 8000 Utopia Pkwy, Jamiaca, NY, 11439, United States
4 - Yale University, 21 Sachem Street, New Haven, CT, 06511, United States
Keywords:
phylogeny
Dipsacales
Lonicera
CUP-SHAPED COTYLEDON
fusion
NAC.
Presentation Type: Poster
Session: P, Systematics Posters
Location: Virtual/Virtual
Date: Thursday, July 30th, 2020
Time: 5:00 PM Time and date to be determined
Number: PSY025
Abstract ID:781
Candidate for Awards:None |
