| Abstract Detail
Angiosperms353: A new essential tool for plant systematics Lagomarsino, Laura [1], Jabaily, Rachel [2]. Angiosperms353: A new essential tool for plant systematics. The advent of high-throughput methods that allow hundreds of nuclear loci to be sequenced from non-model organisms is among the most important technological advances the systematics community has seen. In particular, targeted sequence capture allows researchers to generate carefully curated datasets of putatively orthologous loci— even from the degraded DNA of herbarium specimens. The recent development of the Angiosperms353 probe set, which targets 353 putatively single copy, phylogenetically informative loci identified from transcriptomes of over 600 angiosperm taxa, significantly reduces the cost of sequence capture projects. Combined with falling per-base sequencing costs, the Angiosperms353 probeset makes generating high-quality phylogenomic datasets cost-effective, while removing the need to generate taxon-specific genomic resources ahead of sequence capture. This also reduces the bioinformatic capacity necessary to generate and analyze phylogenomic datasets. Of arguably greater importance, this probe set can be universally applied across angiosperms and the loci they isolate have phylogenetic utility across scales. Together, the cost, ease of analysis, and universality of the Angiosperms353 probeset make it a particularly useful tool for scientists working in clades without extensive genomic resources or in labs without substantial bioinformatic infrastructure.
In addition to convenience for any specific research group, the Angiosperms353 loci are poised to revolutionize angiosperm systematics. Our research community has long sought singular molecular tools that require minimum customization for specific taxa and that generate variable data to resolve both deep nodes and recent radiations across angiosperms. Many exemplar loci, including the reliable rbcL gene, have not fulfilled this goal. However, if our community broadly adopts the Angiosperms353 loci, we can generate continually growing datasets of hundreds of standard loci that can be analyzed with a variety of phylogenetic methods, including those methods that rely on a diversity of gene tree topologies or genome-wide SNP information. This has the potential to lead to new insights in angiosperm relationships at the deepest and at the most recalcitrant nodes, while simultaneously facilitating phylogenetic research at the species-level.
In this symposium, speakers will provide background about the development of the Angiosperms353 probeset and explore how it is currently being used in different clades and at different phylogenetic depths. In addition, speakers will address the utility of the probeset from many different perspectives, including at primarily undergraduate institutions, in the context of international collaborations, when herbarium specimens are the primary source of DNA, and as a global resource set to revolutionize our field.
1 - Louisiana State University, Dept Of Biological Sciences, 103 Life Sciences Building, Baton Rouge, LA, 70803, United States
2 - Colorado College, Department of Organismal Biology & Ecology, 14 E. Cache la Poudre St., Colorado Springs, CO, 80903, USA
Keywords:
none specified
Presentation Type: Symposium Presentation
Session: SY4, Angiosperms353: A new essential tool for plant systematics
Location: Virtual/Virtual
Date: Thursday, July 30th, 2020
Time: 10:15 AM
Number: SY4SUM
Abstract ID:941
Candidate for Awards:None |
